Revision as of 12:07, 25 February 2008

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Tutorial: An Introduction to the Basics of DAS

Introduction

By making use of DAS you can take advantage of being able to view integrated information from multiple sources, without these sources needing to be aware of each other. You can also add your own DAS data source, perhaps privately in your own institution and then view the information served from this source in the context of features from other institutions.

This tutorial will enable you to understand how DAS servers function and how you can find relevant DAS data sources.

Example DAS Reference Server – The UniProt DAS Reference Server

This section will illustrate how the information is provided. Being able to use DAS to full advantage does not require you to understand how the DAS protocol works, however this will give you an insight into how straight forward it can be to develop your own DAS data source and an appreciation of the strengths and weaknesses of DAS.

DAS information is made available from DAS servers. A DAS server is a web application that serves data in the form of a series of XML documents that can be read and processed by DAS client software. There are several different types of XML document, each having a specific function, such as:

• providing the sequence of the molecule (nucleic acid or protein);
• providing details of the features coordinated on a molecule;
• providing a summary of the features coordinated on a molecule.

If features on a specific protein are being obtained from several different locations, it is very useful to ensure that one reliable, common sequence has been obtained. To achieve this, DAS servers are separated into two kinds:

• Reference servers provide the molecule sequence.
• Annotation servers provide features upon the sequence, such as protein domains, or related to the protein, such as journal references. Annotation servers often refer to a specific reference server as their 'map master', i.e. the server that you should expect to be able to retrieve the corresponding sequence from.

You will now investigate an example of each kind of server.

The UniProt DAS Reference Server

The UniProt DAS reference server has the primary purpose of providing sequence information to DAS clients from the UniProt Knowledge Bank. It can be queried using UniProt accession numbers, Swiss-Prot protein Ids and also IPI (International Protein Index) accessions.

Navigate to http://www.ebi.ac.uk/das-srv/uniprot/das/dsn Here you will find a summary page describing the UniProt DAS server and listing the data sources it provides. (If you look at the source of the page, you will see that the browser view is actually an XSLT transformation of the DAS XML returned by the dsn command. The use of this technology with DAS servers is becoming increasingly common.)

Now navigate to http://www.ebi.ac.uk/das-srv/uniprot/das/uniprot/sequence?segment=Q14974

You are now presented with a very simple XML file that contains the sequence of the protein Q14974 (Importin beta-1 subunit). This is the primary function of the UniProt DAS server. Note that the server also indicates the length of the protein as an integer and provides a version in the form of an MD5 digest of the sequence. This is used to allow DAS clients to allow a check that all the DAS sources are referring to the same version of the protein sequence as the reference server.

In addition to being a reference server, the UniProt DAS server also acts as an annotation server, allowing the client to query details of features in the UniProt knowledge bank. This also incorporates InterPro features.

Browse to http://www.ebi.ac.uk/das-srv/uniprot/das/uniprot/features?segment=Q14974

Take a careful look at the result of this search. You should be able to find positional features (note that the start and end coordinates of each feature are included) including Swiss-Prot annotation, non-positional features such as a description of the protein (with start and end coordinates of 0) and also literature references relating to the protein.

As indicated above, the purpose of DAS is to allow you to retrieve protein information from multiple sources at the same time. An example of a completely separate but compatible DAS annotation server that is able to contribute further annotation of the same protein is given in the next task:

Navigate to http://www.ebi.ac.uk/msd-srv/msdmotif/das/s3dm/features?segment=Q14974

Here you will find additional annotation of the same protein from the MSD Motif database at the EBI. Notice that in this case the version is not the same as the version from the UniProt DAS server for the same protein accession. A typical DAS client should note this discrepancy and warn the user that the protein sequence being annotated is possibly not compatible.

Navigate to http://bioinf.cs.ucl.ac.uk:8000/servlet/pdas.pdasServlet2/das/features?segment=Q14974

This is the annotation server based at UCL in London that also provides features for the same protein. In this case (at the time of writing) the version is the same as the UniProt DAS server.

Clearly there are potentially many different DAS servers that may provide annotation that is useful for you. The next section describes how you can manually find DAS services.

Finding DAS sources – the DAS Registry Service at the Sanger Institute

“The purpose of the DAS registration service is to keep track which DAS services are around, which DAS commands they are understanding and about the coordinate systems of their data.”¹

The DAS Registration Server allows DAS clients to discover DAS services. At the same time it provides an elegant browsable web interface that allows you to search for DAS sources, test their status, examine their reliability and learn more about what they offer. At the time of writing, a total of 383 servers from 47 institutions in 18 different countries are included in the registry.

The following activities will familiarise your with the content of the registry, both the 'human' interface and also the web-service interface that can be queried by DAS clients (and your client code!)

Navigate to The DAS Registration Server

Follow the "list" ... "list sources" menu item at the top of this page. On the resulting page, find all DAS annotation servers that use UniProt as their 'authority'.

Select any one of these services and follow the information link ('i' in a blue circle) to find out details of this server including how reliable it has been recently.

For the same DAS service, use the DAS Registration Server to test its current capabilities to find out if it is supporting all of the functionality that it claims to at the present time. (Start with the green tick)

To be added:

view-source: http://www.dasregistry.org/das1/sources

view-source: http://www.dasregistry.org/das1/sources/DS_109

http://www.dasregistry.org/coordsys/CS_DS91

http://www.dasregistry.org/listServices.jsp

http://www.dasregistry.org/listProjects.jsp

For details of web services and WSDL: http://www.dasregistry.org/help_scripting.jsp